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Elek test on The Elek Medium Bacterial toxigenicity was determined by the standardized Elek test on the Elek medium as described previously.20 A half milliliter of Newborn Calf Serum (Sigma) mixture with 2.5 ml Elek medium base at 50-55 oC and poured into the 4.5 cm petri dish, trimmed, and waited until it became solid.
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Endüstriyel elek seçimi nasıl yapılır? Cevher ve Kömür Hazırlama Tesislerinde çok sayıda Elek kullanılır. Bu elekler, dairesl titreşimli elek, doğrusal titreşimli elek, susuzlandırma eleği, muz elek, elek'tir. Elek tipi doğru seçilmezse, tesis kapasitesi düşer, üretim kayıpları artar, elde edilen ürünlerin ...
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This study aims to develop a new modified medium for both Cystinase and Elek tests simultaneously. Methods: There were ten reference strains of bacteria used for the modified medium optimization. Moreover, 15 clinical isolates were used as samples in the modified medium testing. The result of Cystinase and Elek tests on the modified medium was ...
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and the Elek test, respectively [8]. The bacterial strains were grown on Columbia Blood Agar (Oxoid, Basingstoke, UK) for 24 h prior to testing. 2.2. Sample Preparation ELISA testing was performed between February 2021 and January 2022. For ELISA testing bacterial strains were cultured on Elek broth [6,7] for 6 h at 37 C, after …
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A total of 135 strains of E. colo were tested by the SLT Elek test (100 SLT-I-negative and 35 SLT-I-positive). The results of the SLT Elek test and the Vero cell test correlated well: 30 strains gave positive results and 100 strains gave negative results in both tests.
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Purpose: Diphtheria, still present in many countries of the world, is caused by toxigenic strains of species of the Corynebacterium diphtheriae complex, mainly Corynebacterium diphtheriae and the emerging zoonotic pathogen C. ulcerans. The immunoprecipitation test according to Elek is the gold standard for detection of the major virulence factor …
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Elek test procedures, including the use of newborn bovine (calf) serum and diphtheria antitoxin (ADS), were similar to those used in the standard method (6). The results of the identification of bacterial toxigenicity using the standard Elek test and CBAB can be seen inFigure 1. The toxigenic-ity of the diphtheria-causing bacteria and non ...
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A complete convergence of the conventional method of DT detection - Elek test and the LFIA for all 250 isolates of diphtheria and non-diphtheria corynebacteria was observed. The use of the LFIA makes it possible to significantly simplify DT detection and reduce the time of analysis from 24-48 to 4-6 hours. Thus, the possibility of replacing the ...
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This method, described in 1949 by Elek [3] has replaced the in vivo diphtheria virulence test in guinea pigs or rabbits. A modication of the Elek test was proposed by the Ukrainian scientists Feldman et al. [4 ]. This method diers by the use of antitoxin-impregnated paper discs around which bacterial cultures were inoculated in rounded plaques.
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The detection of toxigenicity among Corynebacterium diphtheriae and Corynebacterium ulcerans strains is the most important test for the microbiological diagnosis of diphtheria. Difficulties with current methods, in particular the Elek test, are well documented. We therefore describe a modified Elek …
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metode Elek test . Pemeriksaan elek test dilakukan dengan media elek test pada petri disk diameter 4,5 cm. Disk antitoksin berisi 25 IU ADS (Biofarma) diletakkan di bagian tengah petri, kemudian isolat C.diphtheriae diinokulasi pada bagian tepi dengan jarak 1 cm dari disk antitoksin. Inkubasi pada suhu 37o C selama 24-48 jam.7,9,10.
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Definition of the ELEK's Test: Infection by Corynebacterium diphtheriae is diagnosed using a throat swab. However, the swab contains a mixture of Corynebacterium diphtheriae organisms and normal flora throat organisms. An ELEK test is used to differentiate Corynebacterium diphtheriae organisms from normal flora organisms also called …
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Abstract. A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The sensitivity of the modified Elek test was almost the same as that of the Kanagawa phenomenon on Wagatsuma medium, and the results of both ...
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Elek topluluu elle ve ya mekanik olarak belirli bir ekilde ve belirli bir sre sarslr. Her elek zerinde toplanan madde topluluu alnp tartlr. ... Elek Tesis Documents. Documents · · kÍmyasal numijne hazirlama (ferro alasim) kirma ÖcÜtme sarsak elek (45-150 mtkron) elek (20 kg'a kadar) elek (20 kg - 99 kg) elek (100kg - 199 kg ...
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Bu önemli malzemeyi çıkarmak için Hindistan'ın Keonjhar kasabasında Kleemann'ın MOBISCREEN EVO mobil elek tesisleri kullanılmaktadır: Hindistan merkezli şirket Kalinga Commercial Corporation, Ocak 2020'den bu yana Keonjhar'da altı mobil elek tesisi kullanıyor. Bu da şirketin iddialı üretim hedefine ulaşmasına katkı sağladı.
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